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The effect of fixation on monoclonal antibody labelling on cell surface antigens in cutaneous tissue
Author(s) -
CERIO R.,
BELTER SILKE V.,
MACDONALD D.M.
Publication year - 1987
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1987.tb01890.x
Subject(s) - dermatopathology , medicine , university hospital , dermatology , family medicine
Summary The effect of six fixatives on the immunohistochemical labelling of cell surface antigens in cutaneous tissue was investigated. Following acetone and ethanol fixation of tissue blocks, immunoreactivity was diminished within 20 min at room temperature but only after 4 h at 4°C, compared with unfixed cryostat sections. Formal saline, 3% paraformaldehyde and periodate‐lysine‐paraformaldehyde also permitted immunolabelling up to 4 h even at room temperature. Antigen demonstration was enhanced when the tissue was fixed in ammonium sulphate‐based fixative al room temperature for a minimum of 48 h, although this enhancement was seen up to 7 days. Post‐fixation of fresh‐frozen sections with formal saline for up to 2 h produced no loss of immunoreactivity and preservation of morphological detail was improved.