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The effect of fixation on monoclonal antibody labelling of cell surface antigens in cutaneous tissue
Author(s) -
CERIO R.,
BELTER S.,
MACDONALD D.M.
Publication year - 1987
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1987.tb01869.x
Subject(s) - dermatopathology , medicine , university hospital , dermatology , library science , family medicine , computer science
Summary The effect of six fixatives on the immunohistochemical labelling of cell surface antigens in cutaneous tissue was investigated. Following acetone and ethanol fixation of tissue blocks, immunoreactivity was diminished within 20 min at room temperature hut only after 4 h at 4°C, compared with unfixed cryostat sections. Formal saline 3% paraformaldehyde and periodate‐lysine‐paraformal‐dehyde also permitted immunolabelling after up to 4 h, even at room temperature. Antigen demonstration was enhanced when the tissue was fixed in an ammonium‐sulphate based fixative at room temperature for a minimum of 48 h, although this enhancement was seen for up lo 7 days. Post‐fixation of fresh frozen sections with formal saline for up to 2 h produced no loss of immunoreactivity and preservation of morphological detail was improved.