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Culture grafted leg ulcers
Author(s) -
LEIGH I.M.,
PURKIS P.E.
Publication year - 1986
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1986.tb00526.x
Subject(s) - medicine , dermatology , university hospital , surgery
Summary The culture of keratinocytes from normal skin was attempted for many years but was largely hampered by a tendency for keratinocytes to differentiate in vitro rather than proliferate, and problems with fibroblast overgrowth. However, the report from Rheinwald and Green (1975), which utilizes a mouse 3 T 3 substrate and mitogens, led major advances in culture techniques permitting serial passaging of keratinocyte cultures without fibroblast contamination. Other systems using defined media (Boyce & Ham, 1983) and additives such as epidermal growth factor (Rheinwald & Green, 1977) and bovine pituitary extract (Peehl & Ham, 1980) provide expanded growth without a feeder system. This ability to expand, greatly, a given population of keratinocytes is of obvious clinical application in the repair of epidermal defects and already has been used in the treatment of young burns patients in the States (O'Connor et al. , 1981). We report the use of cultured skin in the grafting of an elderly patient with stasis ulceration.