Premium
In situ differentiation of lymphocytes in light and electron microscopy employing peroxidase‐labelled Helix pomatia lectin. Application for isolated cells and fixed tissues from lymph nodes and cutaneous lymphoma
Author(s) -
TROST T.H.,
WEIL H.P.
Publication year - 1983
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1983.tb01782.x
Subject(s) - helix pomatia , microbiology and biotechnology , biology , lymph , pathology , lymphocyte , lectin , receptor , immunology , medicine , biochemistry , ecology , snail
Summary Peroxidase‐labelled Helix pomatia lectin (HPL), contrasted by histological counterstaining, enables a phenotypic and morphological differentiation of lymphocytes in formaldehyde‐ fixed tissues and cell smears. The HPL receptor was found on 71·5% of peripheral lymphocytes; granulocytes, macrophages and monocytes were negative. Double incubation assays with monoclonal antibodies revealed 97·1±2% of E‐rosette + cells were HPL + and 90·3±3·5% of HPL + lymphocytes were simultaneously OKT 3 + . In cytolysis studies, 8·67plusmn;4·1% of the cells were HPL + and anti‐human Lyt3 − , and 5·8±13% of peripheral blood lymphocytes were HPL + and OKT3. In human lymph nodes, the majority of HPL + lymphocytes was found in the paracortical regions, HPL cells were located in lymphocytic follicles. In mycosis fungoidcs skin infiltrates (plaque stage), HPL + lymphocytes predominated. Electron microscopy revealed homogeneously distributed HPL‐receptor molecules on the cell surfaces of living lymphocytes in human lymph nodes. The HPL‐receptor is thus proven to be related to the T lymphocyte subpopulation, and to provide a useful parameter for in situ lymphoid cell differentiation.