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In situ characterization of tissue lymphoid cells of cutaneous infiltrates using specific membrane antigens
Author(s) -
SCHMITT DANIEL,
ALARIO ANNA,
THIVOLET J.
Publication year - 1979
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1979.tb01614.x
Subject(s) - pathology , antigen , lymphocyte , spleen , sarcoidosis , lymphatic system , frozen section procedure , lymphoma , discoid lupus erythematosus , population , immunology , biology , lupus erythematosus , medicine , antibody , environmental health
Summary A method for identification of B and T lymphocytes on frozen tissue sections is described. The technique was initially established on mouse spleen. Thymodependant cells were identified by anti‐Thy‐i and anti‐theta (alloantigens specific for mouse T lymphocytes) sera and thymoindependant cells by Fab‐Po anti‐Ig serum. This technique was subsequently applied to a study of benign and malignant cutaneous lymphoid infiltrates. Specific membrane markers, human B‐lymphocyte antigens (HBLA) for B lymphocytes and human T‐lymphocyte antigens (HTLA) for T lymphocytes, were revealed by peroxidase immunolabelling. A prominent T‐Iymphocyte population was observed in the cutaneous lesions of sarcoidosis, lichen planus, halo naevus and lupus erythematosus. In contact dermatitis, the prominence of the T‐cell infiltrate is in accordance with the delayed hypersensitivity reaction. Immunoenzymatic labelling of sections of epidermotropic malignant cutaneous lymphoma confirmed the T‐cell nature of the intraepidermal neoplastic cells. This technique of direct specific labelling on sections should also be applicable to electron microscopy.