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A new method for recovery of exudates from normal and inflamed human skin *
Author(s) -
KOBZA BLACK A.,
GREAVES M.W.,
HENSBY C.N.,
PLUMMER N.A.,
EADY R.A.J.
Publication year - 1977
Publication title -
clinical and experimental dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.587
H-Index - 78
eISSN - 1365-2230
pISSN - 0307-6938
DOI - 10.1111/j.1365-2230.1977.tb02560.x
Subject(s) - radioimmunoassay , exudate , histamine , chromatography , bioassay , chemistry , bulla (seal) , prostaglandin , human skin , suction blister , kinin , basal (medicine) , epidermis (zoology) , pathology , endocrinology , medicine , biology , biochemistry , anatomy , lung , bradykinin , genetics , receptor , insulin
Summary A new method for recovery of exudate from human skin using a suction bulla technique is described. Undiluted exudate was obtained with minimal trauma and analysed for histamine, kinin and prostaglandin (PG)‐like activity, by superfusion cascade bioassay. The PG‐like activity was further characterized by gel partition, gas–liquid and thin‐layer chromatography. PGE 2 and PGF 2α were further characterized by gas chromatography–mass spectrometry (GC–MS) and PGF 2α by radioimmunoassay. Histamine was detected as was kinin‐like activity. Prostaglandins E 2 and F 2α with their metabolites, were also detected. The cell content of the bulla fluid was low compared to blood and the protein content was approximately 24 g/1. Histological and electron microscopic studies showed the bullae to arise from a split in the dermo–epidermal junction between the plasma membrane of the epidermal cells and the basal lamina.