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Component‐resolved diagnosis with commercially available D . pteronyssinus D er p 1, D er p 2 and D er p 10: relevant markers for house dust mite allergy
Author(s) -
Bronnert M.,
Mancini J.,
Birnbaum J.,
Agabriel C.,
Liabeuf V.,
Porri F.,
Cleach I.,
Fabre A.,
Deneux I.,
Grandné V.,
Grob J.J.,
Berbis P.,
Charpin D.,
Bongrand P.,
Vitte J.
Publication year - 2012
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2012.04035.x
Subject(s) - immunoglobulin e , immunology , house dust mite , allergy , sensitization , mite , medicine , allergen , asthma , pyroglyphidae , atopic dermatitis , antibody , biology , botany
Background Commercially available house dust mite components may improve routine testing of allergic patients. Objective To establish the prevalence and serum levels of IgE to commercial D er p 1, D er p 2, D er p 10 and the carbohydrate MUXF 3 in house dust‐mite allergic patients. To compare individual vs. allergen microarray methods. Methods Prevalence and serum levels of IgE to D ermatophagoides pteronyssinus extract and components D er p 1, D er p 2, D er p 10 and MUXF 3, specific IgG 4 to D . pteronyssinus , total serum IgE levels, and clinical features (age, asthma, rhinitis and atopic dermatitis) were determined in 123 patients (64 children) with the Immuno CAP ® method. Immuno CAP ISAC ® was performed in 24 patients. Results All patients had serum IgE to D . pteronyssinus . Prevalences of serum IgE to commercial components were D er p 1 93%, D er p 2 77% ( D er p 1 or D er p 2 94%), D er p 10 28% and MUXF 3 25%. Levels of D . pteronyssinus IgE strongly correlated with D er p 1 and D er p 2 IgE ( r = 0.89 and 0.85 respectively), but not D er p 10 and MUXF 3. Immuno CAP ® and Immuno CAP ISAC ® were concordant, but the quantitative correlation was poor. No clinical implication for the prevalence, levels, or molecular IgE reactivity profile to house dust mite components was found. Conclusions and Clinical Relevance Commercially available D er p 1 and D er p 2 strongly correlate with IgE D . pteronyssinus . The lack of D er p 1 and D er p 2 IgE may help with differential diagnosis. D er p 10 serum IgE prevalence and levels suggest different patterns in food and mite‐related tropomyosin sensitization. Serum IgE to carbohydrate MUXF 3, although unexpectedly prevalent, were low and did not modify D . pteronyssinus IgE levels. Follow‐up may be best carried out with individual rather than microarrayed components.