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The LINA cohort: indoor chemical exposure, circulating eosinophil/basophil ( E o/ B ) progenitors and early life skin manifestations
Author(s) -
Weisse K.,
Lehmann I.,
Heroux D.,
Kohajda T.,
Herberth G.,
Röder S.,
Bergen M.,
Borte M.,
Denburg J.
Publication year - 2012
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2012.04024.x
Subject(s) - immunology , peripheral blood mononuclear cell , medicine , progenitor cell , allergy , eosinophil , exhaled breath condensate , cotinine , atopic dermatitis , asthma , biology , stem cell , biochemistry , genetics , in vitro , nicotine
Background Hematopoietic progenitor cells, especially those committed to the E o/ B lineage, are known to contribute to allergic inflammation. Objective The aim of the present study was to investigate whether environmental factors are associated with changes in numbers of circulating E o/ B progenitors at 1 year of age. Methods Peripheral blood from 60 1‐year‐old children enrolled in the LINA (Lifestyle and environmental factors and their Influence on Newborns Allergy risk) birth cohort was assessed for E o/ B progenitor cells ( E o/ B CFU ) using standardized and validated methylcellulose assays. Frozen peripheral blood mononuclear cells ( PBMC ) were cultured in the presence of IL ‐3, IL ‐5 or GM ‐ CSF , and E o/ B CFU s enumerated. Clinical outcomes and exposure to environmental tobacco smoke ( ETS ) were documented by standardized questionnaires, and indoor volatile organic compound ( VOC ) concentrations were assessed by passive sampling. Results Children with skin manifestations (atopic dermatitis or cradle cap) within the first year of life had higher numbers of circulating IL ‐3‐, IL ‐5‐ or GM ‐ CSF ‐stimulated E o/ B CFU s ( P < 0.05) at 1 year. In children with cradle cap, a positive correlation was found between E o/ B CFU s and exposure to ETS ‐related VOC s during pregnancy or at 1 year of age ( P < 0.05). Conclusions and Clinical Relevance This is the first demonstration that environmental exposures are positively associated with levels of circulating E o/ B progenitors. The recruitment and differentiation of E o/ B progenitors in response to environmental triggers may play a role in the development of skin manifestations during the first year of life.