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Reduced T‐bet in addition to enhanced STAT 6 and GATA 3 expressing T cells contribute to human allergen‐induced late responses
Author(s) -
Eifan A. O.,
Furukido K.,
Dumitru A.,
Jacobson M. R.,
SchmidtWeber C.,
Banfield G.,
Durham S. R.,
NouriAria K. T.
Publication year - 2012
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2012.04003.x
Subject(s) - allergen , gata3 , immunology , allergy , medicine , microbiology and biotechnology , biology , biochemistry , transcription factor , gene
Summary Background T‐bet and GATA ‐3 are transcriptional factors involved in Th1 and Th2 cell differentiation, although their concomitant roles at protein levels in target organs during human allergic disease have not been assessed. Objectives We investigated the expression of T‐bet and GATA ‐3 in nasal and cutaneous models of Th2 (grass‐pollen allergen) and a cutaneous model of Th1 ( PPD ) responses in man. Methods Nasal biopsies were obtained at 8 h and skin biopsies at 8 and 48 h after allergen and PPD challenges, respectively, from 10 allergic rhinitics and 6 non‐atopic controls. T cells were assessed using immunofluorescence microscopy. Results There were increases in CD 3 + STAT 6 + cells ( P = 0.01 for nose and skin) and CD 3 + GATA 3 + cells ( P = 0.03 for skin) in response to allergen compared with diluent in allergics. When compared with non‐atopics after allergen challenge the difference between the two groups was also significant for CD 3 + STAT 6 + ( P = 0.001 and 0.03) and for CD 3 + GATA 3 + cells ( P = 0.04 and 0.001) for nose and skin respectively. Following PPD challenge CD 3 + STAT 4 + cells and CD 3 + T‐bet + cells increased in both groups compared with diluent ( P = 0.02 and 0.03 for both TF s), whereas only CD 3 + T‐bet + cells were significantly greater in non‐atopics compared with allergics ( P = 0.04). The ratio of GATA 3 + :T‐bet + T cells in allergen‐induced responses was significantly greater in the allergics ( P = 0.008 and 0.01 nose and skin respectively), whereas the ratio of T‐bet: GATA 3 + T cells was significantly higher in the non‐atopics during PPD ‐induced responses ( P = 0.003). Conclusions and Clinical Relevance Dysregulation of Th1 transcription may contribute to heightened expression of STAT 6 and GATA 3 leading to exaggerated Th2‐driven manifestations of allergic disease.