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High expression of CD 98 alters epithelial barrier functions to promote induction of airway allergy
Author(s) -
Liu T.,
Ma J.,
Li T.L.,
Yang J.F.,
Liang X.,
Yang P.C.
Publication year - 2012
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2012.03978.x
Subject(s) - epithelium , allergy , respiratory epithelium , immunology , barrier function , mucous membrane of nose , thymic stromal lymphopoietin , nasal polyps , medicine , biology , pathology , asthma , microbiology and biotechnology
Summary Background Epithelial barrier dysfunction is critical in the induction of allergy; the aetiology is to be further understood. A recent report indicates that CD 98 plays a role in the intestinal epithelial barrier dysfunction. Objectives This study aimed to investigate the role of overexpression of CD 98 in the induction of nasal allergy. Methods The nasal epithelium samples were collected from 30 patients with allergic rhinitis and 30 healthy subjects. The contents of CD 98 and Staphylococcal enterotoxin B ( SEB ) in the nasal epithelium samples were evaluated by using Western blotting. The effect of SEB of inducing the expression of CD 98 was evaluated with an airway epithelial cell line, the 16 HBE 14o cells. The epithelial barrier function was assessed with the indicators of transepithelial resistance ( TER ) and permeability to horseradish peroxidase ( HRP ). A mouse model was employed to evaluate the role of CD 98 in the induction of nasal allergy. Results High levels of CD 98 and SEB were detected in the nasal epithelium of patients with allergic rhinitis. A positive correlation was identified between CD 98 and SEB in nasal epithelium samples. Exposure to SEB could induce the overexpression of CD 98 in RPMI 2650 and 16 HBE 14o cells. The overexpression of CD 98 down‐regulated TER and increased the permeability to HRP in 16 HBE 14o monolayers. Concurrent exposure to SEB and OVA induced nasal allergies in a mouse model that could be blocked by pre‐treatment with anti‐ CD 98 antibody. Conclusions and Clinical Relevance CD 98 plays a critical role in compromising the airway epithelial barrier function that contributes to the induction of airway allergy.