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12‐hydroxy‐eicosatetraenoic acid (12‐HETE): a biomarker of Churg‐Strauss syndrome
Author(s) -
Szczeklik W.,
Sanak M.,
Mastalerz L.,
Sokołowska B. M.,
Gielicz A.,
Soja J.,
Kumik J.,
Musiał J.,
Szczeklik A.
Publication year - 2012
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2011.03943.x
Subject(s) - exhaled breath condensate , asthma , hypereosinophilic syndrome , eicosanoid , medicine , biomarker , gastroenterology , chemistry , eosinophilia , arachidonic acid , biochemistry , enzyme
Summary Background Churg‐Strauss syndrome ( CSS ) shares similarities with asthma and hypereosinophilic syndrome ( HES ). Eicosanoids – important inflammatory and signaling molecules – are present in exhaled breath condensate ( EBC ) and broncho‐alveolar lavage fluid ( BALF ). Objectives To assess eicosanoid profile both in EBC and BALF of CSS subjects searching for a pattern characteristic of this syndrome. Methods EBC s from 23 CSS patients, 30 asthmatics, 12 HES patients and 54 healthy controls ( HC ) were assessed quantitatively for 19 eicosanoids by a high‐performance liquid chromatography ‐ tandem mass spectrometry ( HPLC ‐ MS / MS ). In addition, in 21 of 23 CSS subjects and in nine asthmatics, eicosanoids were determined in BALF . Results EBC from CSS patients showed markedly elevated levels of 12‐ HETE as compared with other studied groups. BALF was characterized by a significant elevation of 12‐ HETE and its metabolite 12‐tetranor HETE in CSS as compared with asthma. Clinical activity of CSS correlated with 12‐ HETE and its metabolites levels in BALF , but not in EBC . Conclusion and Clinical Relevance CSS is clearly distinguished from bronchial asthma, and HES by a marked increase in 12‐ HETE concentration in both EBC and BALF . This points to a possible new pathogenic mechanism in CSS and may help in future in establishing the diagnosis of CSS .