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Allergen extract‐induced interleukin‐10 in human memory B cells inhibits immunoglobulin E production
Author(s) -
Milovanovic M.,
Heine G.,
Zuberbier T.,
Worm M.
Publication year - 2009
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2009.03233.x
Subject(s) - immunoglobulin e , allergen , immunology , antibody , house dust mite , antigen , interleukin 4 , biology , desensitization (medicine) , chemistry , microbiology and biotechnology , allergy , immune system , receptor , biochemistry
Summary Background Elevated specific IgE antibody levels are common in atopic individuals, caused by T‐helper type 2‐dominated B cell activation. The induction of antigen‐specific IL‐10 secreting T cells is discussed as an important mechanism during specific immunotherapy. By contrast the presence and function of B cell‐derived IL‐10 is not well defined yet. Objective We investigated whether type‐I allergen extracts induce IL‐10 expression in human B cells and analysed its functional role on IgE production. Methods Human peripheral B cells were stimulated with grass pollen, house dust mite (HDM) ( Dermatophagoides pteronyssimus ; Der p) and dog allergen extract. Expression of IL‐10 by activated human B cells was determined by flow cytometric analysis and ELISA. Functional analysis considering immunoglobulin production was assayed by ELISA. Results The allergen extracts studied induced IL‐10 expression in B cells. However, the ability to induce IL‐10 differed between the allergen extracts. The most potent allergen extract was dog (169±28 pg/mL), followed by grass pollen (141±10 pg/mL) and HDM allergen (125±11 pg/mL). Upon allergen extract stimulation only CD27 expressing memory B cells produced IL‐10 and co‐expressed the very early activation antigen CD69. The addition of allergen extracts to B cells activated by anti‐CD40 and IL‐4 selectively inhibited IgE which was dependent on allergen extract‐induced IL‐10. By contrast the other immunoglobulin subclasses like IgA, IgG or IgM were not altered upon allergen extract challenge. Conclusion Our data indicate that allergen‐activated memory B cells can modulate IgE production through secretion of IL‐10.