Premium
Reduced membrane bound CD14 expression in the cord blood of infants with a family history of allergic disease
Author(s) -
Boyle R. J.,
Morley R,
Mah LJ,
Kivivuori S.,
Tang M. LK
Publication year - 2009
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2009.03227.x
Subject(s) - immunology , cord blood , cd14 , immune system , peripheral blood mononuclear cell , medicine , tlr2 , foxp3 , innate immune system , umbilical cord , allergy , biology , biochemistry , in vitro
Summary Background Infants at increased risk of allergic disease have altered immune function at birth, but the specific immune changes described differ between studies and their precise nature is not well defined. Changes affecting innate immune responses may be particularly important in allergic disease pathogenesis. Objective We investigated whether inherited risk of allergic disease is associated with altered markers of innate immunity, T cell regulation or dendritic cell (DC) percentage in human newborns. Methods Cord blood was collected from infants at low risk (no parent affected by allergic disease, n =14), intermediate risk (one affected parent, n =54) or high risk (two affected parents, n =25) of developing allergic disease. Cord blood mononuclear cells were cultured with ovalbumin (OVA), lipopolysaccharide, lipoteichoic acid (LTA), heat‐killed lactobacillus, α‐CD3 or medium alone. Cells were analysed by flow cytometry for expression of CD14, FoxP3 and DC percentage, and by real‐time RT‐PCR for TLR2 and TLR4 expression in infants at intermediate or high risk of allergic disease. Results Infants at high risk of allergic disease had reduced percentage of CD14 + monocytes ( P =0.01) and reduced CD14 mean fluorescence intensity ( P =0.01) in uncultured mononuclear cells. They also had decreased DC percentage in mononuclear cells cultured with OVA ( P =0.04), lipopolysaccharide ( P =0.01), LTA ( P =0.02) and α‐CD3 ( P =0.03) as compared with infants at intermediate or low risk of allergic disease. No relationship was seen between risk of allergic disease and TLR2 or TLR4 expression, or FoxP3 expression in cultured cells. Conclusions Infants with a biparental history of allergic disease have altered markers of innate immunity at birth, with reduced expression of membrane bound CD14 and consequently reduced in vitro development of DCs. Further work is needed to understand the role that these alterations play in the pathogenesis of allergic disease, and whether interventions to up‐regulate fetal CD14 expression can prevent allergic disease.