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Soluble human leucocyte antigen‐G and interleukin‐10 levels in isocyanate‐induced asthma
Author(s) -
Mapp C. E.,
Ferrazzoni S.,
Rizzo R.,
Miotto D.,
Stignani M.,
Boschetto P.,
Maestrelli P.,
Baricordi O. R.
Publication year - 2009
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2009.03215.x
Subject(s) - occupational asthma , asthma , medicine , immunology , peripheral blood mononuclear cell , asymptomatic , lipopolysaccharide , allergy , isocyanate , cytokine , antigen , chemistry , in vitro , biochemistry , organic chemistry , polyurethane
Summary Background We previously reported that in moderate‐to‐severe asthma there is a deficit of IL‐10 secretion that could prevent the production of soluble HLA‐G (sHLA‐G), a non‐classical human leucocyte antigen class I molecule with tissue‐protective properties in inflammatory responses. Objective Our objective was to investigate the production of sHLA‐G and the secretion of IL‐10 by peripheral blood mononuclear cells (PBMCs) in asthma induced by isocyanates and to compare the results with those obtained in non‐occupational allergic asthma. Method sHLA‐G and IL‐10 were measured by ELISA in the culture supernatants of unstimulated or lipopolysaccharide (LPS)‐stimulated PBMCs obtained from 20 subjects with isocyanate asthma, 16 asymptomatic subjects exposed to isocyanates, 18 subjects with non‐occupational allergic asthma, and 26 healthy control subjects. Results Occupational exposure to isocyanates was associated with high baseline levels of secretion of IL‐10 by PBMCs, whether or not the exposed subjects had asthmatic symptoms. However, spontaneous production of sHLA‐G by PBMC was significantly higher in subjects with isocyanate asthma compared with asymptomatic‐exposed controls. In contrast, PBMCs from subjects with non‐occupational allergic asthma produced sHLA‐G only after LPS stimulation. Conclusions sHLA‐G production and IL‐10 secretion are influenced by workplace exposure to isocyanates and by development of asthma. The different behaviour of both sHLA‐G and IL‐10 in asthma induced by isocyanates compared with non‐occupational allergic asthma suggests a heterogeneous biological role for HLA‐G molecules and for IL‐10, a key cytokine of immune and inflammatory responses.