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Localization and function of histamine H 3 receptor in the nasal mucosa
Author(s) -
Suzuki S.,
Takeuchi K.,
Majima Y.
Publication year - 2008
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2008.03041.x
Subject(s) - submucosal glands , thioperamide , mucous membrane of nose , nasal glands , histamine , immunohistochemistry , secretion , medicine , receptor , cholinergic , histamine h3 receptor , endocrinology , pathology , biology , chemistry , agonist
Summary Background Histamine is an important chemical mediator of allergic rhinitis (AR). Histamine H 3 receptors (H 3 R) are located on cholinergic and NANC neurons of the myenteric plexus, and activation of H 3 R regulates gastric acid secretion. However, little is known about the localization and function of H 3 R in the upper airway. Objective The objective of this study was to examine the localization and possible function of H 3 R in the nasal mucosa. Methods We extracted total RNA from the inferior turbinate mucosa of patients with AR. H 3 R mRNA and β‐actin mRNA were amplified by RT‐PCR. We used immunohistochemistry to examine localization of H 3 R protein in the inferior turbinate mucosa excised during clinically indicated surgery. We used alcian blue/periodic acid‐shiff staining to examine the effects of the H 3 R agonist (R)‐α‐methylhistamine and the H 3 R antagonist thioperamide on secretion from rat submucosal glands. Results H 3 R protein was expressed around submucosal gland cells. Thioperamide induced degranulation in the submucosal gland in the nasal septum. Conclusion The present results suggest that H 3 R is localized mainly around submucosal glands, and that H 3 R plays an important role in the secretion of submucosal glands in the nose.