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Human γδ T cells modulate the mite allergen‐specific T‐helper type 2‐skewed immunity
Author(s) -
Korematsu S.,
Tanaka Y.,
Nagakura T.,
Minato N.,
Izumi T.
Publication year - 2007
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2007.02826.x
Subject(s) - immunology , allergen , immunity , mite , biology , allergy , immune system , ecology
Summary Background γδ T cells have been described as one of immune regulators in patients with infection, malignancy, and allergy. Objective To elucidate the ability of γδ T cells as an allergen immunotherapy candidate, the effectiveness of human γδ T cells in allergen‐specific T‐helper type 2 (Th2)‐type T cells was evaluated in vitro . Methods House dust mite‐specific Th2‐type T cell clones, Bacillus Calmette–Guerin (BCG)‐specific Th1‐type T cell clones, and γδ T cell lines were established from the peripheral blood mononuclear cells of two patients with allergic rhinitis. The effectiveness of γδ T cells and BCG‐specific Th1‐type T cell clones in the modulation of allergen‐specific Th2 cells in terms of their cytokine productions was evaluated. Results In response to cognate antigens, the γδ T cell lines demonstrated a proliferation and production of IFN‐γ that exceeded that of BCG‐specific Th1‐type T cell clones (mean stimulation index: 14.5 vs. 2.8, mean IFN‐γ: 130.5 vs. 10.0 pg/mL). When the γδ T cell lines and mite‐allergen‐specific Th2 clones were co‐cultured with each other, only the levels of IL‐4 (mean, −87%) decreased, but not the levels of IL‐5 and IL‐13, with an increasing concentration of γδ T cell antigen and IFN‐γ production (mean, +730%). Conclusion These results demonstrated that γδ T cells derived from allergic patients might thus have a partial ability to modulate allergen‐specific Th2‐skewed immunity.