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Spatial expression of two anti‐inflammatory mediators, annexin 1 and galectin‐1, in nasal polyposis
Author(s) -
Sena A. A. S.,
Provazzi P. J. S.,
Fernandes A. M.,
Cury P. M.,
Rahal P.,
Oliani S. M.
Publication year - 2006
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2006.02570.x
Subject(s) - annexin , biology , galectin 3 , annexin a2 , mast cell , galectin , annexin a1 , immunocytochemistry , inflammation , pathology , epithelium , immunology , microbiology and biotechnology , cancer research , medicine , flow cytometry
Summary Background There is renewed interest in the role played by specific counter‐regulatory mechanisms to control the inflammatory host response, poorly investigated in human pathology. Here, we monitored the expression of two anti‐inflammatory mediators, annexin 1 and galectin‐1, and assessed their potential link to glucocorticoids' (GCs) effective control of nasal polyposis (NP). Methods Total patterns of mRNA and protein expression were analysed by quantitative real‐time PCR (qPCR) and Western blotting analyses, whereas ultrastructural immunocytochemistry was used for spatial localization and quantification of each mediator, focusing on mast cells, eosinophils and epithelial cells. Results Up‐regulation of the annexin 1 gene, and down‐regulation of galectin‐1 gene, was detected in polypoid tissue compared with nasal mucosa. Patient treatment with betamethasone augmented galectin‐1 protein expression in polyps. At the cellular level, control mast cells and eosinophils displayed higher annexin 1 expression, whereas marked galectin‐1 immunolabelling was detected in the granule matrix of mast cells. Cells of glandular duct epithelium also displayed expression of both annexin 1 and galectin‐1, augmented after treatment. Conclusion Mast cells and epithelial cells appeared to be pivotal cell types involved in the expression of both annexin 1 and galectin‐1. It is possible that annexin 1 and galectin‐1 could be functionally associated with a specific mechanism in NP and that GC exert at least part of their beneficial effects on the airway mucosa by up‐regulating, in a specific cell target fashion, these anti‐inflammatory agonists.