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Relationship between neurokinin 2 receptor gene polymorphisms and serum vascular endothelial growth factor levels in patients with toluene diisocyanate‐induced asthma
Author(s) -
Ye Y.M.,
Kang Y.M.,
Kim S.H.,
Kim C.W.,
Kim H.R.,
Hong C.S.,
Park C.S.,
Kim H.M.,
Nahm D.H.,
Park H.S.
Publication year - 2006
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2006.02547.x
Subject(s) - toluene diisocyanate , single nucleotide polymorphism , asthma , vascular endothelial growth factor , haplotype , genotype , medicine , immunology , occupational asthma , methacholine , respiratory disease , biology , gene , lung , chemistry , vegf receptors , biochemistry , organic chemistry , polyurethane
Summary Background Among the various pathogenic mechanisms of toluene diisocyanate (TDI)‐induced asthma, a contribution from neurogenic inflammation has been suggested. Objective To evaluate neurokinin 2 receptor (NK2R) gene polymorphisms in association with the clinical phenotype of TDI‐induced asthma, 70 TDI‐induced occupational asthma (TDI‐OA)patients, 59 asymptomatic exposed controls (AEC), and 93 unexposed healthy controls (NC) were enrolled in the study. Methods Two single‐nucleotide polymorphisms (SNPs) of NK2R, 7853G>A (Gly231Glu) and 11 424G>A (Arg375His), were genotyped using a single base extension method. The levels of PC 20 methacholine, specific IgE and IgG to TDI–human serum albumin conjugate, and serum vascular endothelial growth factor (VEGF), matrix metalloproteinase‐9, and TGF‐β1 were compared according to the NK2R genotypes of the subjects with TDI‐OA and AEC. Results No significant differences in allele, genotype, or haplotype frequencies of these two SNPs were noted among the three groups ( P >0.05, respectively). Moreover, subjects with the NK2R 7853GG genotype had higher serum VEGF levels than those with GA or AA among the TDI‐exposed workers ( P =0.040). Conclusion The NK2R 7853GG genotype may contribute to increased serum VEGF levels, which result in airway inflammation after TDI exposure.