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Lipopolysaccharide exposure makes allergic airway inflammation and hyper‐responsiveness less responsive to dexamethasone and inhibition of iNOS
Author(s) -
Komlósi Z. I.,
Pozsonyi É.,
Tábi T.,
Szökő É.,
Nagy A.,
Bartos B.,
Kozma G. T.,
Tamási L.,
Orosz M.,
Magyar P.,
Losonczy G.
Publication year - 2006
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2006.02514.x
Subject(s) - eosinophil , immunology , dexamethasone , ovalbumin , bronchoalveolar lavage , inflammation , medicine , eosinophil peroxidase , lipopolysaccharide , cytokine , allergic inflammation , glucocorticoid , immune system , lung , asthma
Summary Allergic airway disease can be refractory to anti‐inflammatory treatment, whose cause is unclarified. Therefore, in the present experiment, we have tested the hypothesis that co‐exposure to lipopolysacharide (Lps) and allergen results in glucocorticoid‐resistant eosinophil airway inflammation and hyper‐responsiveness (AHR). Ovalbumin (Ova)‐sensitized BALB/c mice were primed with 10 μg intranasal Lps 24 h before the start of Ova challenges (20 min on 3 consecutive days). Dexamethasone (5 mg/kg/day) was given on the last 2 days of Ova challenges. AHR, cellular build‐up, cytokine and nitrite concentrations of bronchoalveolar lavage fluid (BALF) and lung histology were examined. To assess the role of iNOS‐derived NO in airway responsiveness, mice were treated with a selective inhibitor of this enzyme (1400W) 2 h before AHR measurements. More severe eosinophil inflammation and higher nitrite formation were found in Lps‐primed than in non‐primed allergized mice. After Lps priming, AHR and concentrations of T‐helper type 2 cytokines in BALF were decreased, but still remained significantly higher than in controls. Eosinophil inflammation was partially, while nitrite production and AHR were observed to be largely dexamethasone resistant in Lps‐primed allergized animals. 1400W effectively and rapidly diminished the AHR in Ova‐sensitized and challenged mice, but failed to affect it after Lps priming plus allergization. In conclusion, Lps inhalation may exaggerate eosinophil inflammation and reduce responsiveness to anti‐inflammatory treatment in allergic airway disease.