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The DR4–DQ8 haplotype and a specific T cell receptor Vβ T cell subset are associated with absence of allergy to Can f 1
Author(s) -
Kinnunen T.,
Taivainen A.,
Partanen J.,
Immonen A.,
Saarelainen S.,
RytkönenNissinen M.,
Rautiainen J.,
Virtanen T.
Publication year - 2005
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2005.02247.x
Subject(s) - t cell receptor , immunology , allergy , t cell , flow cytometry , haplotype , human leukocyte antigen , t lymphocyte , peripheral blood mononuclear cell , immunoglobulin e , antigen , biology , allergen , microbiology and biotechnology , genotype , medicine , antibody , immune system , genetics , gene , in vitro
Summary Background The significance of specific T cell receptor (TCR) Vβ subtypes and human leucocyte antigen (HLA) class II alleles for the development of allergy to lipocalin allergens such as the major dog allergen Can f 1 is not clear at present. Objective To characterize the TCR Vβ usage in the Can f 1‐specific T cell lines and the HLA class II genotypes of Can f 1‐allergic and non‐allergic subjects. Methods T cell lines were induced with recombinant Can f 1 from the peripheral blood mononuclear cells of 12 non‐atopic dog owners and 26 dog‐allergic patients. Thirteen of the dog‐allergic subjects were sensitized to Can f 1. Expression of the TCR Vβ subtypes on CD4 + T cells in the T cell lines was measured by flow cytometry. The subjects were HLA genotyped for DRB1, DQB1 and DPB1 loci. Results Can f 1‐specific T cell lines were obtained from 18 subjects, with either positive ( n =8) or negative ( n =10) skin prick tests (SPTs) to recombinant Can f 1. The frequency of TCR Vβ5.1 + T cells was significantly higher in the T cell lines of subjects with negative SPTs to the allergen. Moreover, DR4–DQ8 haplotype was over‐represented among these subjects. Conclusion The DR4–DQ8 haplotype and the TCR Vβ5.1 + CD4 + T cells may be protective against allergy to Can f 1.