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Prevalence of sensitization to Artemisia allergens Art v 1, Art v 3 and Art v 60 kDa. Cross‐reactivity among Art v 3 and other relevant lipid‐transfer protein allergens
Author(s) -
Lombardero M.,
GarcíaSellés F. J.,
Polo F.,
Jimeno L.,
Chamorro M. J.,
GarcíaCasado G.,
SánchezMonge R.,
DíazPerales A.,
Salcedo G.,
Barber D.
Publication year - 2004
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.2004.02053.x
Subject(s) - mugwort , plant lipid transfer proteins , sensitization , artemisia , cross reactivity , immunoglobulin e , allergen , immunology , allergy , population , medicine , chemistry , traditional medicine , cross reactions , antibody , biochemistry , pathology , alternative medicine , environmental health , gene
Summary Background Artemisia vulgaris is a widespread weed in the Mediterranean area and several allergens have been detected in its pollen. One of them, Art v 3, belongs to the lipid‐transfer protein (LTP) family and its prevalence in Artemisia ‐sensitized patients or its relationship with other LTP allergens is not clear. Objective To assess the pattern of sensitization to an array of mugwort allergens in a Mediterranean population, and to study the cross‐reactivity of Art v 3 with Pru p 3 and Par j 1, relevant LTP allergens in the area. Methods Skin prick test was performed with whole extracts ( A. vulgaris, Parietaria judaica and peach) and pure natural allergens Art v 1, Art v 3, Art v 60 kDa and Par j 1 in 24 mugwort‐allergic patients from a Mediterranean area. In vitro assays included measurement of specific IgE and ELISA inhibition among LTP allergens. Results The three Artemisia allergens elicited a positive skin response in 70–80% of the patients. Seven patients were clearly sensitized to Par j 1 and 11 to Pru p 3. There was no correlation between Par j 1 and Pru p 3 sensitization, but a highly significant correlation was found between peach extract and Art v 3 as regards the skin response. No IgE cross‐reactivity was observed between Art v 3/Par j 1 or Pru p 3/Par j 1. In contrast, Art v 3 significantly inhibited the binding to Pru p 3 of IgE from three patients' sera out of six studied, but Pru p 3 was not able to inhibit the IgE binding to Art v 3. Conclusion Art v 3 is a major mugwort allergen and in some patients with IgE to both Art v 3 and Pru p 3, Art v 3 behaves as the primary sensitizing agent.