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Substance P is generated in vivo following nasal challenge of allergic individuals with bradykinin
Author(s) -
BAUMGARTEN C. R.,
O'CONNOR A.,
DOKIC D.,
SCHULTZ K. D.,
KUNKEL G.
Publication year - 1997
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1997.tb01178.x
Subject(s) - bradykinin , in vivo , substance p , pharmacology , chemistry , medicine , neuropeptide , biology , receptor , genetics
Summary Background Bradykinin, a potent inflammatory mediator, is released during allergic and non‐allergic rhinitis and asthma in man. Nasal bradykinin challenge induces a dose‐dependent plasma leakage into the nasal cavity and relevant symptoms of rhinitis. Objective We now report on substance P generation during nasal bradykinin challenge in vivo . Methods The effect ot locally applied bradykinin on substance P generation was studied in nine individuals, allergic to grass pollen and six non‐allergic controls. In the allergies TAME‐esterase activity, histamine and substance P concentrations were measured in nasal lavages and correlated to the clinical symptoms. Results Substance P concentrations in nasal lavages increased in a dose‐dependent fashion during nasal bradykinin challenge in both groups. In the allergic group Substance P‐increases correlated with the production of TAME‐esterase activity ( r = 0.9. P < 0.05) whereas these allergic individuals did not produce any histamine increases. The generation of substance P and the increase of TAME‐esterase activity was associated with the onset of clinical symptoms. Correlation of oedema and hypersecretion to substance P were signiticant by linear regression analysis ( r = 0.88, P < 0.005 and r = 0.89. P < 0.02, respectively). Bradykinin induced irritations like burning and itching were shortterm and rare. Serial dilutions of nasal washes produced Substance P‐RIA displacement curves that paralleled the standard curve and recovery of standard substance P that was added to nasal washes was 76 ± 4% (mean ± sem), n = 8. Conclusion Bradykinin induces in vivo a dose‐dependent plasma leakage into the nasal cavity without affecting mast cells, but stimulates nerve endings resulting in the release of the neuropeptide substance P.