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Damage to respiratory epithelium by guinea‐pig eosinophils stimulated with IgG‐coated Sepharose beads
Author(s) -
HIRATA A.,
MOTOJIMA S.,
FUKUDA T.,
MAKINO S.
Publication year - 1996
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1996.tb00617.x
Subject(s) - percoll , respiratory epithelium , epithelium , guinea pig , microbiology and biotechnology , respiratory tract , chemistry , immunology , biology , respiratory system , pathology , medicine , centrifugation , biochemistry , endocrinology , anatomy
Summary Background There are no reports of respiratory epithelial damage induced by immuiioglobulin‐stimulated eosinophils. Objective We tried to induce damage to respiratory epithelium by guinea‐pig (GP) eosinophils stimulated with guinea‐pig IgG (GP‐lgG)‐coated Sepharose 4B beads. Methods GP tracheal epithelium was cultured together with GP eosinophils that had been collected from the peritoneal cavity, purified on a Percoll gradient, and stimulated with GP‐lgG‐coated beads (GP eosinophils:beads = 20:1). Damage to the epithelium was observed with an inverted microscope. Results After 24 h of culture with three 10 6 eosinophils. irregularity of the surface of the epithelium, desquamation. shedding of cilia, and abnormal beating of cilia were observed. This damage was first observed after 12 h of incubation, and was more severe at 24 h. No damage was found when beads coated with human serum albumin (HSA) were used. GP eosinophils stimulated with GP‐IgG released significantly more EPO than those stimulated with HSA. at 6 and 24 h. Heparin and eatalase partially inhibited the epithelial damage. O 2 ‐production by eosinophils was also enhanced with GP‐IgG‐coated beads. Conclusion Both granule basic proteins and reactive oxygen radicals may be responsible for epithelial damage, probably via an EPO + H 2 O 2 + halide system. These results confirmed that stimulated eosinophils can damage respiratory epithelium.