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Immunohistochemical detection of GM‐CSF, IL‐4 and IL‐5 in a murine model of allergic bronchopulmonary aspergillosis
Author(s) -
CHU H. W.,
WANG J. M.,
BOUTET M.,
BOULET L. P.,
LAVIOLETTE M.
Publication year - 1996
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1996.tb00563.x
Subject(s) - allergic bronchopulmonary aspergillosis , immunohistochemistry , aspergillosis , medicine , immunology , pathology , aspergillus fumigatus , immunoglobulin e , antibody
Summary Background Granulocyte macrophage‐colony stimulating factor (GM‐CSF), interleukin‐4 (IL‐4) and IL‐5 are important in tissue eosinophil accumulation and high IgE production in allergic inflammatory reaction. Objective We examine lung GM‐CSF, IL‐4 and IL‐5 expression in a murine model of allergic bronchopulmonary aspergillosis (ABPA) characterized by eosinophil and lymphocyte lung infiltration and elevated serum IgE level. Methods C57BL/6 mice were intranasally treated three times a week for 1, 2 or 3 week(s) with Aspergillus fumigatus (Af) antigen or saline and were sacrificed on days 7, 14 and 21. Immunohistochemical analyses for GM‐CSF, IL‐4 and IL‐5 were performed on lung sections. Results Af treatment induced a remarkable pulmonary eosinophil influx. Increased numbers of lung T lymphocytes and GM‐CSF positive cells were observed on days 14 and 21. IL‐4 and IL‐5 positive cells were increased significantly only on day 14. Immunostained serial sections showed that most (≥98%) cytokine positive cells were CD3 positive. Few eosinophils (<2% of cytokine positive cells) were immunoreactive for GM‐CSF and IL‐5. Significant correlations were found between the number of GM‐CSF and IL‐5 positive cells, and the number of eosinophils in Af‐treated lung (r = 0.62, P < 0.05 and r = 0.52, P < 0.05, respectively), and between the number of IL‐4 positive cells and the serum total IgE level (r = 0.64, P<0.01). Conclusions Our data suggest a role for T lymphocyte GM‐CSF, IL‐4 and IL‐5 in Af‐induced mouse pulmonary eosinophilia and increased serum IgE production and further support the importance of T helper (TH) cells in the pathogenesis of ABPA.