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Decreased production of IFN γ and increased production of IL‐6 by cord blood mononuclear cells of newborns with a high risk of allergy
Author(s) -
LIAO S. Y.,
LIAO T. N.,
CHIANG B. L.,
HUANG M. S.,
CHEN C. C.,
CHOU C. C.,
HSIEH K. H.
Publication year - 1996
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1996.tb00555.x
Subject(s) - cord blood , immunology , peripheral blood mononuclear cell , immunoglobulin e , atopy , medicine , allergy , cd8 , cytokine , cd16 , monocyte , cd3 , biology , immune system , antibody , biochemistry , in vitro
Summary Background The underlying mechanisms of elevated IgE level in atopic patients are still obscure, however, extensive efforts have been tried to identify an immunological parameter as a predictor of atopy. Objective This study compared the difference in cytokine production by cord blood mononuclear cells between new borns with high‐risk of allergy (family allergy score, FAS ± 3) and those with low‐risk (FAS = 0). Methods Following stimulation with PHA(100 μg/mL) and PMA (1 ng/mL), the cytokines produced by cord blood CD4 + T cells in the presence of monocytes were measured by ELISA kits and the mRNA was detected by reverse transcription‐polymerase chain reaction (RT‐PCR) technique. Results Our results showed: CD4 + T cells in the presence of monocytes and isolated monocytes from the high‐risk group produced a much greater amount of IL‐6, either spontaneously or after stimulation, than did those of the low‐risk group; CD4 + T cells of low‐risk group produced a significantly greater amount of interferon gamma (IFNγ) than did those from the high‐risk group; IL‐4 cannot be detected by ELISA kit, and only a trace amount of IL‐4 mRNA was detected by RT‐PCR technique; cord blood basophils stimulated with PHA and PMA could produce a significant amount of IL‐4; there was an inverse correlation between the production of IFNγ and cord blood IgE level (high‐risk group, r ‐ 0.647, n = 17) and the number of natural killer (NK) cells (CD3 − CD16 + CD56 + ) was signiticantly lower in high‐risk group than for low‐risk group. Conclusion Our data suggested increased production of IL‐6 and decreased production of IFNγ of cord blood mononuclear cells appear to be the hallmark of newborns from the high‐risk population.