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Detection of Pityrosporum orbiculare reactive T cells from skin and blood in atopic dermatitis and characterization of their cytokine profiles
Author(s) -
LINDER M. TENGVALL,
JOHANSSON C.,
ZARGARI A.,
BENGTSSON Å.,
PLOEG I.,
JONES I.,
HÄRFAST B.,
SCHEYNIUS A.
Publication year - 1996
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1996.tb00526.x
Subject(s) - atopic dermatitis , peripheral blood mononuclear cell , immunology , immunoglobulin e , malassezia , antibody , cytokine , radioimmunoassay , biology , allergy , microbiology and biotechnology , in vitro , endocrinology , biochemistry
Summary Background Atopic dermatitis (AD) is associated with increased levels of serum igE. and T‐helper (Th) cells are thought to a play role in the pathogenesis. Individuals with AD often develop IgE antibodies against the yeast Pityrosporum orhiculare . a member of the normal cutaneous flora. Objective The role of P. orbiculare in atopic dermatitis was investigated by examining the T‐cell reactivity for P. orbiculare . Methods Freshly isolated peripheral blood monoruclear cells (PBMC) were isolated from 10 AD patients with serum IgE antibodies against P. orbiculare , and from six healthy controls. The proliferative response after P. orbiculare stimulation, measured by [ 3 H]thymidine incorporation, was examined in the PBMC and in T‐cell clones (TCC) obtained from skin and blood of one patient. The cytokine profile of the TCC was determined by enzyme‐linked immunosorhent assay (ELISA). radioimmunoassay (RIA) and reverse transcriptase‐polymerase chain reaction (RT‐PCR) following challenge with either P. orbiculare extract or anti‐CD3 antibodies and phytohaemag‐glutinin. Results The PBMC response to P. orbiculare was significantly higher in the AD patients than in the control group ( P < 0.05). Twenty‐nine out of 36 tested TCC derived from one responding patient were reactive for P. orhiculare . The clones were CD 2+ and CD 4+ , except for one CD 8+ blood clone. A majority of the TCC derived from lesional skin showed a Th2‐ or Th2/Th0‐like cytokine profile. A co‐expression of interIeukin‐5 (IL‐5) mRNA and II‐13 mRNA was detected in five out of six P. orhicularc‐reative clones analysed for their cytokinc gene expression with RT‐PCR. Conclusion Our data suggest that P. orhiculare can induce a T‐cell response in AD patients. The Th2‐like profile of P. orbiculare‐reactive TCC derived from lesional skin indicates that P. orhiculare may play a role in maintaining IgE‐mediated skin inflammation in AD.