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Cloning and sequencing of the group 6 allergen of Dermatophagoides pteronyssinus
Author(s) -
BENNETT B. J.,
THOMAS W. R.
Publication year - 1996
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1996.tb00501.x
Subject(s) - allergen , complementary dna , catalytic triad , proteases , cdna library , microbiology and biotechnology , house dust mite , biology , serine , cloning (programming) , chymotrypsin , oligonucleotide , serine protease , molecular cloning , chemistry , trypsin , biochemistry , immunology , enzyme , protease , allergy , gene , computer science , programming language
Summary Background The allergen Der p 6 from Dermatophagoides pteronyssinus has been described by substrate affinity as mite chymotrypsin. Objective The aim of this paper was to describe a cDNA clone encoding the allergen. Methods cDNA was cloned from a λ library using oligonucleotides published for Der p 6. Results The clone P6.1.1 had the N‐terminal amino acid residues as reported for Der p 6, and at position 189 the Ser was conserved in chymotrypsin for substrate specificity as well as the catalytic triad of His57, Asp102 and Ser195 for serine proteases. The estimated Mr was 24.9K and it was 37% identical to the trypsin allergen Der p 3. Conclusion cDNA encoding Der p 6 has been described.