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Human auto‐anti‐idiotypic antibodies to mite‐specific IgE can degranulate human basophils in vitro
Author(s) -
WEYER A.,
MAO J.,
ETIEVANT M.,
DAVID B.,
GUINNEPAIN M. T.,
SAINTREMY J. M. R.
Publication year - 1995
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1995.tb00395.x
Subject(s) - immunoglobulin e , immunology , antibody , in vitro , biology , biochemistry
Summary Background Anti‐idiotypic antibodies (anti‐Ids) to specific IgE antibodies are formed spontaneously during an anti‐allergen immune response and can be induced by immunotherapy. Although anti‐Ids can down‐regulate the production of IgF. antibodies, at least in experimental models, their possible role in the modulation of target cell reactivity remains ill‐defined. Objective The capacity of human anti‐Ids to modulate the release of histamine was examined in an in vitro system of human basophil degranillation. Anti‐Ids were prepared from the serum of six Dermatophagoides pteranyssinus ( DP )‐hypersensitive patients suffering from atopic dermatitis and who had never been desensitized. Basophils were obtained from the blood of atopic donors. The extent of histaminc release was determined using a fluorometric assay. Results We show that: anti‐Ids trigger the release of histamine in an allergen‐specific, dose‐ and IgE‐dependent manner; the release is not due to the presence of allergen and/ or anti‐IgE antibodies: and that the degranulating activity can be removed by absorption with affinity‐purified anti‐ Dp antibodies of the corresponding patient. Conclusion These results indicate that spontaneously produced human anti‐Ids can modulate the reactivity of human basophils.