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Leukotriene B 4 production by blood neutrophils in allergic rhinitis—effects of cetirizine
Author(s) -
CHERIASAMMARI S.,
ALOUI R.,
GORMAND F.,
CHABANNES B.,
GALLET H.,
GROSCLAUDE M.,
MELAC M.,
RIHOUX J. P.,
PERRINFAYOLLE M.,
LAGARDE M.,
PACHECO Y.
Publication year - 1995
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1995.tb00010.x
Subject(s) - cetirizine , leukotriene b4 , immunology , ex vivo , priming (agriculture) , cytokine , leukotriene , in vivo , allergy , in vitro , medicine , chemistry , pharmacology , inflammation , biology , asthma , biochemistry , botany , germination , microbiology and biotechnology
Summary Background: Mucosal inflammatory processes in late phase of allergic diseases involve cytokine production, cell adhesion molecule overexpression and release of inflammatory mediators with chemotactic activity, such as leukolriene B 4 (LTB 4 ), We had previously observed increased production of LTB 4 by neutrophils in patients with allergic rhinitis and discussed the role of granulocyte macrophage‐colony stimulating factor (GM‐CSF) priming. Some antihislaminic compounds were shown to diminish the production of leukotrienes by neutrophils. Objective: In a first step, we evaluated in ex vivo and in vitro studies, the effects of cetirizine on LTB 4 production by blood neutrophils from allergic and healthy subjects. In a second step, we studied the in vitro effect of cetirizine on LTB 4 production by neutrophils from healthy subjects during GM‐CSF priming of these cells. Methods: Neutrophils from both populations were purified from venous blood and LTB 4 production was measured using high performance liquid cromatography (HPLC) method. Results: In ex vivo studies, cetirizine treatment induced a decreased LTB 4 production by neutrophils in allergic rhinitis. This effect of decreased LTB 4 production was reproduced in vitro with 10 −8 –10 ‐6 cetirizine. Nevertheless, this anti‐Hl compound had no effect on neutrophil priming with GM‐CSF. Conclusion: As LTB 4 is an important chemotactic factor, Cetirizine could act on inflammatory cell recruitment by inhibiting LTB 4 production by neutrophils.