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Bronchial provocation with cat allergen: correlation between the individual IgE‐CRIE pattern and the occurrence of a late allergic reaction
Author(s) -
MOSIMANN B.,
AUBERT V.,
FREY J. G.,
LEUENBERGER PH.,
PÉCOUD A.
Publication year - 1994
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1994.tb00916.x
Subject(s) - provocation test , immunoglobulin e , allergen , immunology , medicine , immunoelectrophoresis , asthma , allergy , dander , radioallergosorbent test , antigen , antibody , pathology , alternative medicine
Summary Twenty‐one mild asthmatic patients allergic to cat dander underwent a bronchial provocation test (BPT) with a cat extract. An early allergic response (EAR) was observed in all 21 patients and a late allergic response (LAR) in 8/21 patients. In the EAR, the patients with subsequent LAR had a greater full in FEV 1 , Their baseline FEV 1 , and total dose of inhaled allergen were not significantly different from patients who did not develop a LAR, but their serum specific IgE level was higher. Crossed immunoelectrophoresis (CIE) of the same cal extract showed that it contained eight different proteins. An IgE‐CRIE was obtained from all 21 patients, using radiolabelled anti‐lgE and autoradiography. Radiolabelled standards allowed a semiquantitative scoring of the radiosiaining. The CRIE pattern of the eight patients with LAR showed a higher score of radiostaining and a greater number of proteins hound to IgE. The two major allergen cal albumin and Fel d I bound equally to IgE of patients with and without LAR whereas another protein (antigen No. 7) bound to IgE of 100% of patients with LAR but of only 38% of patients without LAR. These data suggest that the pattern of the IgE response to specific proteins of a cat extract may be related to the occurrence of LAR after BPT with this allergen.