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Identification of monocyte chemotactic factors in supernatants of ovalbumin‐stimulated lymphocytes from patients with atopic dermatitis who are sensitive to hen's egg
Author(s) -
FUKUTOMI O.,
KONDO N.,
AGATA H.,
SHINODA S.,
KUWABARA N.,
SHINBARA M.,
INOUE R.,
ORII T.
Publication year - 1994
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1994.tb00247.x
Subject(s) - monocyte , ovalbumin , peripheral blood mononuclear cell , chemotaxis , immunology , atopic dermatitis , antigen , biology , medicine , in vitro , biochemistry , receptor
Summary Monocyte chemotactic activities in supernatants of ovalbumin (OA)‐stimulated peripheral blood mononuclear cell (PBMC) cultures were studied in patients with atopic dermatitis (AD) who were sensitive to hen's egg. The monocyte chemotactic activities in hen's egg‐sensitive AD patients were significantly higher than those of non‐atopic healthy controls and patients with immediate allergic symptoms. However, the monocyte chemotactic activities were not detected in bovine serum albumin‐Stimulated PBMC culture supernatants in patients with AD who were sensitive to hen's egg. but not to cow's milk. Furthermore, there was significant correlation between the monocyte chemotactic activities and proliferative responses of PBMCs to OA in hen's egg‐sensitive AD patients, whereas there was no significant correlation between the monocyte chemotactic activities and radioallergosorbent test values. These results suggest that PBMCs stimulated with food antigens produce monocyte chemotactic factors which relate to the pathogenesis of AD in food‐sensitive AD patients and that the pathogdenesis of AD may be related to cell‐mediated immune responses.