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Laser high performance liquid chromatography determination of prostaglandins in nasal lavage fluid in allergic rhinitis
Author(s) -
SUGIMOTO M.,
SUGIYAMA S.,
YANAGITA N.,
OZAWA T.
Publication year - 1994
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1994.tb00242.x
Subject(s) - histamine , nasal lavage , medicine , allergy , bronchoalveolar lavage , nasal administration , chemistry , anesthesia , immunology , lung
Summary This study was designed to analyse prostaglandins (PGs) in human nasal lavage‐ fluid using the combination of microcolumn high performance liquid chromatography and a He/Cd laser induced fluorescence detection system. Forty‐seven patients with allergic rhinitis and 12 healthy volunteers were investigated. Four species of PG. i.e. PGD 2 , PGE 2 , PGF 2x and 6‐keto‐PGF 1 , were detected in the nasal lavage fluid. Concentrations of PGD 2 ; (1.33 ± 0.17 nmol/ml) and PGE 2 (0.87 ± 0.11 nmol/ml) in nasal lavage fluid from patients with allergic rhinitis (the allergy group) were significantly increased compared with those of volunteers (the control group, 0.23 ± 0.16 nmol/ml, 0.29 ±0.19 nmol/ml, respectively). On the other hand, no significant differences were observed in concentrations of cither PGF 2x , or 6‐keto‐PGF 1x between the control group and the allergy group. Histamine concentration in nasal lavage fluid was significantly increased in the allergy group (53 ± 7.6 nmol/l) compared with the control group (3.4 ± 1.0 nmol/1). No significant correlation was observed between PGD 2 and histamine concentration ( r = 0.24), or between PGE 2 and histamine concentration ( r = 0.08) in nasal lavage fluid from patients with allergic rhinitis. Treatment with oxatomide, an anti‐histamine and anti‐allergic drug, significantly improved symptom scores, but did not alleviate them completely. Concentrations of each PG detected in nasal lavage fluid did not change significantly after oxatomide treatment. It is concluded that, not only histamine but also PGs, particularly PGD 2 and PGE 2 , might be involved in the genesis of allergic rhinitis.