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Standardization of pollen allergens of Parthenium hysterophoms and selection of an in‐house reference extract
Author(s) -
SRIRAMARAO P.,
SCHRODER H.,
RAO B. S. SUBBA,
PRAKASH O.,
RAOH P. V. SUBBA
Publication year - 1993
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1993.tb01790.x
Subject(s) - parthenium , ragweed , pollen , allergen , medicine , potency , immunology , allergy , biology , botany , in vitro , biochemistry , weed
Summary A standardized in‐house reference extract from the pollen of Parthenium hysterophoms , which is responsible for the high incidence of allergic rhinitis in India, was generated and examined by skin test, radio‐allergosorbent test inhibition and isoelectric focusing. Parthenium reference allergen discs and positive reference serum were also generated. These reference reagents could not only be used for the quantitation of Panhenium ‐specific IgE in the sera of rhinitis patients but also for the evaluation of allergenic activity (relative potency and lot‐to‐lot variation) of different batches of Parthenium pollen.