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β 2‐ Adrenoceptor stimulation augments the IL‐4‐induced CD23 expression and release and the expression of differentiation markers (CD14, CD18) by the human monocytic cell line, U 937
Author(s) -
PAULEUGENE N.,
DUGAS B.,
GORDON J.,
KOLB J. P.,
CAIRNS J. A.,
PAUBERTBRAQUET M.,
MENCIAHUERTA J. M.,
BRAQUET P.
Publication year - 1993
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1993.tb00329.x
Subject(s) - cd23 , agonist , monocyte , cd14 , salbutamol , endocrinology , medicine , cytokine , receptor , biology , interleukin 4 , cell culture , stimulation , pharmacology , chemistry , immunoglobulin e , immunology , antibody , asthma , genetics
Summary The effect of β 2‐ adrenoceptor agonists and interleukin‐4 (IL‐4) on the CD23 expression on, and release from, the human promonocytic cell line, U 937, was investigated. As assessed by flow cytometry, incubation of U 937 cells in the presence of salbutamol, fenoterol or IL‐4 induced a concentration‐ and time‐dependent increase in CD23 expression, that was maximal after 48 hr and followed by a decrease thereafter. In addition, salbutamol potentiated the effect of IL‐4, the optimal concentration of the drug being a function of the concentration of this cytokine. This synergy between IL‐4 and β 2‐ adrenoceptor agonists was also observed for the release of the soluble form of CD23. The effect on CD23 expression of salbutamol and fenoterol, but not of IL‐4, was blocked in the Wesence of d, l‐propranolol (1 μm) or butoxamine (1 μm). The α‐adrenoceptor agonist, norepinephrine (1 μm), was ineffective in inducing CD23 expression or potentiating the one evoked by IL‐4. Salbutamol down‐regulated the expression of Fc γ RI (CD64) and Fc, RII (CD32) whereas IL‐4 was ineffective. Only when added together at the onset of the culture did salbutamol and IL‐4 induce, after 48 hr, the expression of the monocyte marker, CD 14. The expression of CD 18 was up‐regulated in response to salbutamol either alone or in combination with IL‐4, this cytokine alone being inefficient. These data suggest that IL‐4 and β 2‐ adrenoceptor agonists induce differentiation of U 937 cells into monocyte‐like cells.