Premium
Pistachio nut hypersensitivity: identification of pistachio nut allergens
Author(s) -
PARRA F. M.,
CUEVAS M.,
LEZAUN A.,
ALONSO M. D.,
BERISTAIN A. M.,
LOSADA E.
Publication year - 1993
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1993.tb00290.x
Subject(s) - anacardiaceae , nut , olea , immunoglobulin e , pistacia , polyacrylamide gel electrophoresis , radioallergosorbent test , sensitization , gel electrophoresis , brazil nut , biology , chemistry , botany , horticulture , food science , antibody , biochemistry , immunology , structural engineering , engineering , enzyme
Summary Type I hypersensitivity to pistachio nut antigens was demonstrated in three patients by means of immediate skin‐test reuctivily. specific IgF determination by a lluoro‐immunoassay (CAP). CAP‐inhibition and leucocyte histamine release. Sensitization to other dried fruils and pollens was observed in the patients. The CAP‐inhibition studies revealed signilicLint crossreactivity between pistachio and cashew nut belonging to the Anacardiaceae family, and between pistachio nut and other dried fruits belonging to taxonomically unrelated botanical families. No relevant crossallergenicity was observed between pistachio nut and Lolium and Olca pollens. Sodium dodecyi sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) of a pistachio nut extract followed by immunoblotting analysis identified four IgE‐binding bands with molecular weights of 34. 41. 52 and 60 kD.