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Molecular cloning of a house dust mite allergen with common antibody binding specificities with multiple components in mite extracts
Author(s) -
SHEN HD.,
CHUA KY.,
LIN KL.,
HSIEH KH.,
THOMAS W. R.
Publication year - 1993
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1993.tb00278.x
Subject(s) - allergen , house dust mite , immunoglobulin e , antibody , mite , microbiology and biotechnology , clone (java method) , complementary dna , chemistry , peptide sequence , biology , biochemistry , allergy , immunology , dna , gene , botany
Summary Plaque radio‐immuno assay has been used to isolate an FgE‐binding clone from a lambda gt11 library of Dermatophagoides pteronyssinus cDNA, The clone HD6 contained DNA encoding a 215 residue protein which contained a predicted 17 amino acid residue leader sequence, no cysteines and a single W‐glycosylation site. The 198 residue mature protein would have a predicted MW of 22 177 D. No homologues were found in searches of the data banks. Sera from 14/38 allergic children reacted strongly with the polypeptide produced by the clone (37%). Skin tests showed reactivity in 16/30 (53%) allergic patients and 0/10 of controls. Affinity purification of rabbit antibodies with the clone showed that antibodies to the polypeptide had specificities to multiple products in mite extracts corresponding to components of Mr 29, 27 and 24 K by Western blotting. Absorption studies of IgE in allergic serum indicated further entities at 13 and 11.5 kD, It is proposed to name this allergen Der p VII.