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Monoclonal antibodies bind identically to both spores and hyphae of Aspergillus fumigatus
Author(s) -
REIJULA K. E.,
KURUP V. P.,
KUMAR A.,
FINK J. N.
Publication year - 1992
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1992.tb00164.x
Subject(s) - immunoelectron microscopy , aspergillus fumigatus , monoclonal antibody , microbiology and biotechnology , antigen , hypha , biology , cytoplasm , antibody , conidium , immunology , biochemistry , genetics
Summary Immunoelectron microscopy (IEM) was used to determine the binding of six monoclonal antibodies (MoAbs) produced against Aspergillus fumigatus antigens present on or within the conidia and hyphae of the fungus. Antigen‐antibody complexes were demonstrated in EM using labelled colloidal gold particles (15 nm). Three out of 6 MoAbs (C9, F12 and H10) reacted only with the cytoplasmic components of A. fumigatus while the remaining three (B12, F6G5 and D6E6) showed reactivity to both cytoplasm and cell wall of the conidia and hyphae. The results indicate that IEM is of considerable value in determining and selecting monoclonal antibodies having specific reactivity with diverse antigenic components.