Premium
Group V allergens in grass pollens: IV. Similarities in amino acid compositions and NH 2 ‐terminal sequences of the Group V allergens from Lolium perenne, Poa pratensis and Dactylis glomerata
Author(s) -
KLYSNER S.,
WELINDER K. G.,
LØWENSTEIN H.,
MATTHIESEN F.
Publication year - 1992
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1992.tb00152.x
Subject(s) - phleum , lolium perenne , dactylis glomerata , poa pratensis , lolium , biology , allergen , red clover , botany , poaceae , allergy , immunology
Summary Monoclonal antibodies (PpV4) raised against Phleum pratense group V allergen were used for immuno‐affinity chromatography of cross‐reacting group V allergens from related grass species. Fractions enriched in group V allergen were obtained from Lolium perenne, Poa pratense and Dactylis glomerata extracts. The major components in these fractions were found in the Mw r range 25–28 kD. IgE binding to these components was shown using a pool of grass allergic sera, by SDS‐PAGE immunoblotting. These fractions were electroblotted from tricine SDS‐PAGE gels onto a polyvinylidenedifluoride membrane and selected group V bands were directly cut out and used for amino acid analysis and NH 2 ‐terminal sequencing. Both the amino acid compositions and the NH 2 ‐terminal sequences obtained for each group V allergen were almost similar to each other and to the sequence and composition of the previously described allergen Phl p V from Phleum pratense. A common trait of the investigated allergens, is the very high contents of alanine (25–32%) and the presence of the modified amino acid, hydroxyproline.