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Comparison between MAGIC LITE®‐ and CAP®‐system: two automated specific IgE antibody assays
Author(s) -
KLEINETEBBE J.,
EICKHOLT M.,
GÄTJEN M.,
BRUNNÉE T.,
O'CONNOR A.,
KUNKEL G.
Publication year - 1992
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1992.tb00150.x
Subject(s) - immunoglobulin e , immunology , antibody , medicine
Summary The MAGIC LITE system, a newly developed immunochemiluminometric assay for specific and total IgE antibody using paramagnetic particles coupled with standardized allergens as solid phase, was compared to the CAP system, a recently introduced immunoassay based on a cellulose polymer encased in a capsule. A total of 357 serum samples of patients with suspected inhalant allergies and a positive skin prick test (SPT) to common allergens (birch, timothy‐grass, mugwort, cat dander, Dermatophagoides pteronyssinus , Alternaria) were investigated. Fifty SPT negative subjects served as controls (total number of tests in each assays = 1600). Both assays were highly precise (overall intra‐assay and inter‐assay coefficients of variation were 2.9% and 4.5% in MAGIC LITE, 4.7% and 5.5% in CAP) and showed excellent linearity (mean r 2 of eightfold log 2 serum dilutions were 99.7% and 99.3% in MAGIC LITE and CAP). Good correlations were found between the absolute specific IgE antibody values detected by both methods (correlation coefficient r : birth 0.86, grass 0.93, mugwort 0.96, cat 0.91, D. pteronyssinus 0.73 , Alternaria 0.90). Excellent specificity (.98%) occurred in both assays and with all allergens, and sensitivity was related to the allergen (MAGIC LITE/CAP): birch 91%/89%, grass 83%/90%, mugwort 50%/69%, cat 83%/83%, D. pteronyssinus 72%/78%, Alternaria 75%/81 %. Our results indicate that both in vitro tests are useful tools for the detection of specific IgE antibody.