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Evidence for IgE immune complexes and distribution of IgG subclasses with anti‐IgE activity in patients with atopic dermatitis
Author(s) -
CARINI C.,
FRATAZZI C.,
AIUTI F.
Publication year - 1991
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1991.tb01689.x
Subject(s) - immunoglobulin e , immunology , atopic dermatitis , autoantibody , subclass , medicine , allergy , immune system , radioimmunoassay , antibody
Summary The anti‐IgE autoantibody was detected, using a radioimmunoassay, in 17 out of 35 (48.6%) of patients with atopic dermatitis. Significant increased levels of IgG‐anti‐IgE were seen in the patients studied compared with the control group. The specificity of the anti‐IgE autoantibody was confirmed by competitive inhibition assay using IgG, IgM, IgE myeloma. A correlation was observed between the levels of IgG ‐anti‐IgE and serum IgE but not between the IgG subclasses with anti‐IgE activity and the clinical status. These data demonstrate that the IgG subclass distribution with anti‐IgE activity belongs mostly to the IgG1 and IgG4 subclasses compared with the controls. Moreover, ultracentrifugation analysis indicated that the IgG‐anti‐IgE in the serum samples from the patients with atopic dermatitis was present in the form of an immune complex with self‐IgE. These observations may suggest that the anti‐IgE complexes may play a broader role in the modulation of the immune response and that this autoantibody may mask recognition of IgE in conventional assays.