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cDNA encoding the major mite allergen Der f II
Author(s) -
TRUDINGER M.,
CHUA K. Y.,
THOMAS W. R.
Publication year - 1991
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1991.tb00801.x
Subject(s) - complementary dna , allergen , epitope , homology (biology) , mite , biology , microbiology and biotechnology , glycosylation , molecular cloning , antibody , house dust mite , amino acid , immunology , genetics , gene , allergy , botany
Summary cDNA encoding the major house dust mite allergen Der f II from Dermatophagoides farinae was amplified using the polymerasc chain reaction and cloned into E. coli . It encoded a 129‐residue protein with a calculated molecular weight of 14021 D and had the expected high homology (88%) with Der p II including the absence of N ‐glycosylation sites and conserved cysteine residues. These results are consistent with the high degree of antibody crossreactivity and may help identify the differences in T‐cell epitopes revealed for these molecules so far.