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The allergens of dog. I. Identification using crossed radio‐immunoelectrophoresis
Author(s) -
FORD ANNETTE W.,
ALTERMAN LESLEY,
KEMENY D. M.
Publication year - 1989
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1989.tb02362.x
Subject(s) - antigen , immunoelectrophoresis , dander , immunology , immunoglobulin e , bovine serum albumin , radioallergosorbent test , antibody , medicine , biology , chemistry
Summary The antigens present in an extract of dog hair and dander were examined by crossed immunoelectrophoresis (CIE) and the IgE‐binding allergens by crossed radio‐immunoelectrophoresis (CRIE), respectively, using sera from 60 British and Finnish animal‐allergic subjects. The extract was comprised of a minimum of 28 antigens, 11 of which were common to dog serum. IgE antibody in the sera of the dog‐sensitive patients bound to 21 of the 28 antigens at varying frequencies and intensities. Binding of any intensity occurred most frequently to two serum proteins: antigen 23 (IgG) binding IgE in 88% of cases, and antigen 3 (dog serum albumin, DSA) in 77% of cases. Dander antigen 8 bound in 63% and antigen 1 in 42% of the sera. Strong IgE binding, however, was most commonly associated with dander antigen 8 followed by antigens 1 and 23 (IgG) then 3 (DSA). The ranking of the antigens as allergens was similar for the two populations except that DSA was more important for the British than for the Finnish subjects.