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Effect of hypo‐ and hyper‐osmolarity on basophil histamine release
Author(s) -
RIMMER J.,
BRYANT D. H.
Publication year - 1986
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1986.tb00769.x
Subject(s) - histamine , osmole , basophil , osmotic concentration , mannitol , endocrinology , sodium , medicine , immunology , pharmacology , chemistry , immunoglobulin e , antibody , biochemistry , organic chemistry
Summary The pattern of histamine release from peripheral white blood cells in response to hyper‐(sodium chloride, mannitol) and hypo‐osmolar (water) stimuli was studied in order to determine the differences between non‐atopic control and atopic asthmatic patients in histamine release caused by osmolar changes. All subjects showed histamine release to both hyper‐ and hypo‐osmolar stimuli and this response was reproducible. Viability studies and the effect of time and temperature indicated that, at the extremes of hypo‐ and hyper‐osmolality, histamine release was associated with cell cytotoxicity. Asthmatics and controls showed no differences either in their level of spontaneous or hyper‐osmolar sodium chloride induced histamine release, but asthmatics' cells were more responsive to mannitol over 600–800 mOsm/kg and less responsive to a hypo‐osmolar stimulus under 80 mOsm/kg. This suggests that asthmatics' cells may differ from controls in the manner in which they respond to osmotic stimuli and this may be relevant in certain clinical situations.