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RAST inhibition: a collaborative study on its suitability and reproducibility for the assay of cocksfoot pollen extracts
Author(s) -
SEAGROATT VALERIE,
FORD ANNETTE
Publication year - 1986
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1986.tb00768.x
Subject(s) - radioallergosorbent test , reproducibility , allergen , dactylis glomerata , chromatography , potency , pollen , chemistry , biology , immunology , botany , allergy , in vitro , biochemistry , poaceae
Summary The suitability and reproducibility of the inhibition mode of the radioallergosorbent test (RAST) as a method for the assay of allergen extracts was examined in a collaborative study involving seven laboratories. Participants assayed five coded extracts of cocksfoot grass ( Dactylis glomerata ) pollen, including three batches from one manufacturer, both by a common method and by their local methods of RAST inhibition. For the versions of RAST inhibition employed in this study the estimates of relative potencies of the extracts were in agreement. Further, when laboratories used their local methods, the inter‐laboratory variation in potency estimates was similar to that found when laboratories used the common method. It was concluded that RAST inhibition has a useful role in the assessment of the potencies of allergen extracts, and of the consistency of serial batches of extracts from individual manufacturers.