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In vitro IgE‐secreting cells in man I. Mitogen‐independent and ‐dependent subpopulations
Author(s) -
MATSUMOTO T.,
YAMAGUCHI K.,
SHIMAMURA M.,
HARADA Y.
Publication year - 1985
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1985.tb02256.x
Subject(s) - pokeweed mitogen , immunoglobulin e , mitogen activated protein kinase , in vitro , radioimmunoassay , immunology , biology , antibody , peripheral blood mononuclear cell , endocrinology , biochemistry
Summary In vitro IgE secretion by atopic and normal peripheral‐blood lymphocytes was examined in culture with pokeweed mitogen or Staphylococcus aureus strain Cowan‐I (StaCw) or without mitogen. IgE secreted in culture supernatants was measured with double antibody radioimmunoassay. Enumeration of IgE‐secreting cells was made by a protein‐A plaque assay. IgE was detected in increasing quantities in supernatants of cultured lymphocytes without mitogen up to the 12th day. IgE‐plaque‐forming cells were formed by the lymphocytes in large numbers on days 4–7 in cultures with mitogen. These results suggest that not only mitogen‐independent but also mitogen‐dependent subpopulations may exist in the IgE‐secreting cells.