Premium
Immunoglobulin E antibodies to ingested cereal flour components: studies with sera from subjects with asthma and eczema
Author(s) -
SUTTON R.,
HILL D. J.,
BALDO B. A.,
WRIGLEY C. W.
Publication year - 1982
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1982.tb03127.x
Subject(s) - immunoglobulin e , gliadin , radioallergosorbent test , coeliac disease , antibody , allergy , immunology , isoelectric focusing , globulin , wheat flour , asthma , food allergy , chemistry , medicine , food science , gluten , biochemistry , disease , enzyme
Summary The specificity of immunoglobulin E for different cereal grain proteins was investigated using sera from twenty paedialric patients with asthma and/or eczema. Close correlations were observed between radioallergosorbent test values for grain extracts of wheat, rye and barley, and, to a lesser extent, oats. Of the different wheat flour fractions tested, the globulins and glutenins consistently bound higher levels of IgE than the gliadins and albumins. This is in contrast both with bakers' asthma (an allergy to inhaled flour where the albumins are important allergens) and with coeliac disease (in which gliadin is the most toxic fraction). Partial digestion of the flour proteins largely removed their ability to bind IgE, An analytical technique of identifying allergens after gel isoelectric focusing demonstrated that many different flour proteins were involved.