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Observations on high spontaneous release of histamine from leucocytes in vitro
Author(s) -
MAY C. D.,
REMIGIO LINDA
Publication year - 1982
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1982.tb02523.x
Subject(s) - histamine , in vitro , sensitization , centrifugation , immunology , in vivo , antigen , chemistry , pharmacology , biology , biochemistry , microbiology and biotechnology
Summary Clinical and laboratory observations are provided on high, spontaneous, in vitro histamine release (HSHR) from leucocytes (basophils), meaning without addition of antigen or other inducers of histamine release. HSHR has been observed predominantly in persons with a high degree of reaginic sensitivity to food; the leucocytes of forty‐eight of fifty‐eight individuals (83%), who had positive reactions to foods in double‐blind challenges, exhibited HSHR. HSHR resembles antigenic release in a wide variety of characteristics. HSHR does not occur in vivo but only in vitro after compaction of leucocytes by centrifugation at room temperature. An indispensible requirement for HSHR to occur appears to be a high degree of reaginic sensitization.