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Allergen‐specific IgE and IgG4 antibody levels in sera and the capacity of these sera to sensitize basophil leucocytes in vitro
Author(s) -
TOORENENBERGEN A. W.,
AALBERSE R. C.
Publication year - 1982
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1982.tb01643.x
Subject(s) - immunoglobulin e , basophil , immunology , allergen , antibody , dander , house dust mite , medicine , allergy
Summary Leucocytes from normal non‐allergic donors were passively sensitized with sixty‐five atopic sera that contained IgE directed against house dust mite (HDM) or guinea‐pig dander (GPD). In no serum was there any IgE with an abnormal basophil anaphylactic‐sensitizing activity. In sera from patients hypersensitive to GPD, both GPD‐specific IgE and IgG4 were measured. A significant correlation was found between the GPD‐IgE RAST and the basophil‐sensitizing capacity of GPD‐positive sera, but no correlation was found between this sensitizing capacity and the GPD‐lgG4 RAST. Leucocytes were passively sensitized with serum mixtures that contained the same amounts of total and HDM‐specific IgE; these mixtures differed as to sensitizing activity. It is unclear whether this variation is due to differences in IgE or to influences of other factors in serum.