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Supravital observation of in vitro basophils in immunological reactions
Author(s) -
KIMURA I.,
TANIZAKI Y.,
SATO S.,
TAKAHASHI K.,
SAITO K.,
UEDA N.
Publication year - 1981
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1981.tb01563.x
Subject(s) - immunoglobulin e , basophil , immunology , in vitro , basophil activation , antibody , asthma , medicine , chemistry , biochemistry
Summary The migration velocity and morphology of basophils in vitro were examined after the addition of anti‐immunoglobulin (anti‐lgE or anti‐IgG). In atopic asthma patients with high serum IgE levels (more than 1000 i.u./ml) basophils showed increased migration velocity and showed pear‐shaped and palmate processes after the addition of anti‐lgE, but on the addition of anti‐IgG, these basophils did not show increased migration velocity or morphological changes. In intractable asthma patients with low serum IgE levels, these changes occurred with anti‐IgG but not with anti‐IgE. These in vitro findings suggest a basophil reactivity to anti‐IgE and anti‐IgG in individual patients and support our previous differentiation of asthma patients according to basophil reaction.