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The isolation of an allergen from extracts of Dermatophagoides pteronyssinus using a murine IgA myeloma with a specificity for phosphorylcholine
Author(s) -
STEWART G. A.,
McWILLIAM A.,
TURNER K. J.
Publication year - 1980
Publication title -
clinical and experimental allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.462
H-Index - 154
eISSN - 1365-2222
pISSN - 0954-7894
DOI - 10.1111/j.1365-2222.1980.tb02144.x
Subject(s) - phosphorylcholine , allergen , chemistry , microbiology and biotechnology , radioallergosorbent test , hapten , mite , allergy , immunology , biology , biochemistry , antibody , botany
Summary The presence of a high molecular weight glycoprotein component bearing the hapten phosphorylcholine (Pc) has been demonstrated in some but not all extracts of house dust mite using a phosphorylcholine specific IgA myeloma protein (S107). The Pc bearing component was isolated from house dust mite extracts by precipitation at equivalence using whole myeloma serum and the isolated fraction was found to be allergenic as judged by a direct RAST assay using a highly house dust mite allergic human serum pool. RAST inhibition studies using free hapten and direct RAST studies using pneumococcal polysaccharide C‐substance indicated that the Pe moiety did not contribute significantly to the immunodominant portion of the isolated allergen. Further studies indicated that the S107 isolated material was immunochemically different from the tridacnin reactive material and the con A reactive material previously isolated from extracts of house dust mite.